G1549

PNGase F from Elizabethkingia meningoseptica

ready-to-use solution, recombinant, expressed in E. coli

• Sinónimos: PNGase F
• CAS Number: 83534-39-8
• Número CE: 3.5.1.52 (BRENDA, IUBMB)
• Número MDL: MFCD18839214
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• recombinante: expressed in E. coli
• Nivel de calidad: 200
• conjugado: (N-linked)
• grado: Proteomics Grade
• Formulario: ready-to-use solution
• actividad específica: ≥1000 U/mg
• caducidad: ≥1 yr at -20 °C
• mol peso: ~36 kDa
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Recombinant PNGase F has been purified by affinity chromatography and dialyzed into a 50% glycerol solution with 10 mM potassium phoosphate pH 7.5 to produce a stable product. The product contains low levels of buffer salts. This highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-terminal 6x histidine fusion tag. PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation assay in human plasma samples and in deglycosylation of chondroitin sulfate proteoglycan.

Used to deglycosylate protein.

Acciones bioquímicas o fisiológicas

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGase F from Elizabethkingia meningoseptica has glycan-binding catalytic domain and a bowl-like domain at the N-terminus. It cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It is cost-effectively produced on a large scale in prokaryotic hosts and requires divalent zinc ions for its enzymatic activity.

Definición de unidad

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Forma física

Supplied as 300 Units/mL enzyme in 50% (v/v) glycerol and 50% (v/v) 20 mM Potassium Phosphate, pH 7.5.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable