Recognizes Histone H4 when acetylated on Lys12.

Wide range of cross-reactivity expected based on sequence homology.

Peptide corresponding to Histone H4 containing the sequence [GLG-AcK-GGA] on which Lys12 is acetylated.

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µL of Negative Control Supernatant, or 2 µL of Anti-Acetyl-Histone H4 (Lys12) and the Magna ChIP A Kit (Cat. # 17-610).
Successful immunoprecipitation of Acetyl-Histone H4 (Lys12)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH Coding Region as a positive locus, and a gene desert region as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Lysates from HeLa cells untreated or sodium butyrate treated (Lanes 1 and 2 respectively) were resolved probed with anti-acetyl-Histone H4 (Lys12) (1:1,000). Arrow indicates Acetyl-Histone H4 (Lys12).
Arrow indicates Acetyl-Histone H4 (Lys12) (~11 kDa)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

This Anti-acetyl-Histone H4 (Lys12) Antibody, Trial Size / Pack is validated for use in WB for the detection of acetyl-Histone H4 (Lys12).

Routinely evaluated by immunoblot.

11kDa

10 μL of rabbit monoclonal IgG cell culture supernatant with 0.1% sodium azide.

2 years at -20°C from date of shipment

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