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48-619MAG

Millipore

MILLIPLEX® Total/Phospho Erk - Cell Signaling Multiplex Assay

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20000 UNITS
CA$155.00
100000 UNITS
CA$477.00

About This Item

UNSPSC Code:
12161503
NACRES:
NA.47

CA$155.00

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species reactivity

human, mouse, rat

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex® xMAP®)

storage temp.

2-8°C

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W270709M48805W488401
6-Methyl-5-hepten-2-one natural, ≥98%, FCC

W270733

6-Methyl-5-hepten-2-one

6-Methyl-5-hepten-2-one ≥98%, FCC, FG

W270709

6-Methyl-5-hepten-2-one

6-Methyl-5-hepten-2-one 99%

M48805

6-Methyl-5-hepten-2-one

6-Methyl-5-hepten-2-ol natural, ≥95%, FG

W488401

6-Methyl-5-hepten-2-ol

organoleptic

apple; musty; fruity

organoleptic

apple; musty; fruity

organoleptic

-

organoleptic

coriander; green; sweet

grade

Fragrance grade, natural, Kosher

grade

FG, Fragrance grade, Halal, Kosher

grade

-

grade

FG, Fragrance grade, Halal, Kosher, natural

food allergen

no known allergens

food allergen

no known allergens

food allergen

-

food allergen

no known allergens

documentation

see Safety & Documentation for available documents

documentation

see Safety & Documentation for available documents

documentation

-

documentation

see Safety & Documentation for available documents

assay

≥98%

assay

≥98%

assay

99%

assay

≥95%

agency

follows IFRA guidelines, meets purity specifications of JECFA

agency

follows IFRA guidelines, meets purity specifications of JECFA

agency

-

agency

follows IFRA guidelines

General description

The MILLIPLEX® MAP Total/Phospho Akt (Catalog No. 48-618MAG) and Total/Phospho ERK (48-619MAG) Magnetic bead kits have been developed for the simultaneous detection of phosphorylated Akt (Ser473) and total Akt or phosphorylated ERK1/2 (Thr185/Tyr187) and total ERK in a single well using the Luminex system. These detection assays are rapid, convenient alternatives to Western Blotting and immunoprecipitation procedures for the analysis of cell lysate samples.

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.An overnight (4°C) incubation is recommended for best results.This assay requires 25 μL diluted cell lysate per well.This kit must be run using Assay Buffer 2 (provided).1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL)Analytes available:Erk (Total)Erk (Thr185/Tyr187)

Packaging

96-well plate

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

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    Relationship between sensory and instrumental analysis for tomato flavor.
    Baldwin EA, et al.
    J. Am. Soc. Hort. Sci., 123(5), 906-915 (1998)
    B D Whitaker et al.
    Journal of agricultural and food chemistry, 48(6), 2040-2043 (2000-07-11)
    Conjugated triene (CT) oxidation products of alpha-farnesene have long been thought to be involved in development of superficial scald in apple fruit. Early studies found that CT hydroperoxides and the volatile 6-methyl-5-hepten-2-one (MHO) are major in vitro autoxidation products of
    Thangavel Rajagopal et al.
    Behavioural processes, 85(1), 58-67 (2010-06-16)
    In ungulates the process of chemical communication by urinary scent marking has been directly related to reproductive dominance, territorial defense and proximity to resources. The differences in the frequency of urine marking and chemical composition of urine of males Antelope
    E Sprecher et al.
    Antonie van Leeuwenhoek, 49(4-5), 493-499 (1983-11-01)
    The accumulation of volatile metabolites in cultures of 34 strains comprising ten species of the genus Ceratocystis (Ascomycetes) has been investigated under defined culture conditions. The identified compounds include short-chain alcohols and esters, lower terpenes, terpenoids, and 2-phenylethyl acetate. Certain
    W Reichardt
    Canadian journal of microbiology, 27(1), 144-147 (1981-01-01)
    Certain norcarotenoids, which have recently been found as excretion products of freshwater cyanobacteria and algae, are potent inhibitors of different metabolic functions in heterotrophic bacteria. 6-Methylhept-5-en-2-one showed the strongest effects and acted as a noncompetitive inhibitor of both glucose uptake
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