AB3150

Anti-β-Actin (ACTB) Antibody

CHEMICON^®, rabbit polyclonal

• Synonym(s): 32 kDa Fragment of beta-Actin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• Product Name: Anti-Fractin Antibody, CT, serum, Chemicon^®
• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI accession no.: NM_001101.2
• UniProt accession no.: P60709
• shipped in: dry ice
• target post-translational modification: unmodified

Description

General description

During apoptosis, beta actin is cleaved by activated caspase 3 between Asp244 and Gly245. This cleavage results in the release of an approximately 130 amino acid (15 kDa) fragment from the C-terminus of beta actin and a 32 kDa (244 amino acid) N-terminal fragment of actin, known as Fractin. The C-terminus of the Fractin fragment represents an apoptosis-specific neoepitope that is not antigenic in native full-length beta actin and therefore presents a novel and specific marker for distinguishing apoptotic from necrotic cells.

Specificity

By immunohistochemistry and Western blot, this antibody only recognizes the 32 kDa Fractin fragment but not full-length beta actin.

Immunogen

K-YELPD peptide representing the last five amino acids of the C-terminus of the 32 kDa Fractin fragment of beta-Actin. This sequence is absolutely conserved in Fractin fragments from human, mouse, rat, feline, porcine, ovine, bovine, canine, rabbit and guinea pig origin.

Application

Detect Fractin using this Anti-Fractin Antibody, C-terminus validated for use in WB, IC, IH(P).

Immunohistochemistry/Immunocytochemistry: 1:400-1:1000. 1 hr at 37°C or 4°C overnight. Note: If studying paraffin-embedded tissue, the use of antigen retrieval methods is recommended.

Western Blot: 1:1000-1:4000 for incubation overnight at 4°C. Recommended antibody dilution buffer: TBS with 3% BSA and 0.05% Tween 20, pH 7.4

Optimal working dilutions must be determined by end user.

Physical form

Liquid whole rabbit serum containing no preservatives.

Storage and Stability

Aliquot and store at -20°C for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.

Analysis Note

Control
POSITIVE CONTROL: Staurosporin treated neuronal cultures.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable