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AB7821

Sigma-Aldrich

Anti-Collagen Type VI Antibody

serum, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

NCBI accession no.

UniProt accession no.

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This Item
SAB4500388SAB4500389AB765P
Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

serum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

serum

UniProt accession no.

P12109

UniProt accession no.

P12110

UniProt accession no.

P12111

UniProt accession no.

P11087

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

technique(s)

ELISA: suitable, immunohistochemistry: suitable

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:40000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100

technique(s)

ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable

Specificity

Human collagen type I <0.5%

Human collagen type II <0.5%

Human collagen type III <0.5%

Human collagen type IV <0.5%

Human collagen type V 5%

Human collagen type VI 100%

Human fibronectin <0.5%

Human albumin <0.5%

Human immunoglobulin <0.5%

Immunogen

Human placental collagen type VI

Application

Anti-Collagen Type VI Antibody is an antibody against Collagen Type VI for use in ELISA, IH.
Immunohistochemistry: 1:10-1:40 dilution of reconstitute for indirect immunofluorescent staining of frozen tissue sections.

ELISA: 1:10,000 dilution of reconstitute for ELISA on human collagen type VI.

Optimal working dilutions must be determined by the end user.

Physical form

Pooled antisera was cross-absorbed against immobilized human serum proteins and collagen types IV and V. The IgG fraction was then precipitated with ammonium sulfate and subsequently dialyzed. Lyophilized from 500μL of 10mM Na-phosphate, 150mM NaCl, pH 7.5 with 0.1% mannitol.

Storage and Stability

Maintain lyophilized at -20°C for up to 12 months. Reconstitute with 500uL of distilled water or saline. Store reconstitute between 2 and 8°C for up to two months. Remove any insoluble material by microcentrifugation before use.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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