Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC 1.14.13.8; EC 1.14.13.148; UniProt P31513; also known as Dimethylaniline oxidase 3, FMO form 2, FMO 3, FMO II, Hepatic flavin-containing monooxygenase 3, Trimethylamine monooxygenase) is encoded by the FMO3 (also known as FMOII, TMAU) gene (Gene ID 2328) in human. Flavin-containing mono-oxygenases (FMOs) constitute a family of microsomal chemical- and drug-metabolizing enzymes, with FMO3 being a major form in adult mouse and human liver. FMO3 is responsible for N-oxidation of a malodorous metabolite, trimethylamine (TMA), to the non-odorous trimethylamine N-oxide (TMAO). FMO3 mutations cause trimethylaminuria (TMAU or Fish odor syndrome), a rare inherited disorder due to decreased metabolism of dietary-derived TMA. More than 300 single nucleotide polymorphisms of the human FMO3 have been reported and over 40 of these polymorphisms have been linked to TMAU. 17β-estradiol-dependent ERα activation is reported to play a fundamental role in the regulation of FMO3 gene transcription.

Epitope: Internal (N-terminal half).

Linear peptide corresponding to an internal sequence from the N-terminal half of human FMO3.

Detect FMO3 using this Anti-FMO3 Antibody validated for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected FMO3 in human liver tissue.

Research Category
Signaling

Research Sub Category
Toxicology & Drug Resistance

Evaluated by Western Blotting in human liver microsomal tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected FMO3 in 10 µg of human liver microsomal tissue lysate.

~55 kDa observed. 59.96 kDa calculated.

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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