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MAB1397Z

Sigma-Aldrich

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free

clone 3E2B, Chemicon®, from hamster(Armenian)

Synonym(s):

CD54, MAB1397

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20 μG
MYR 741.00
50 μG
MYR 1,323.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

MYR 741.00

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biological source

hamster (Armenian)

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

3E2B, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

NM_000201.1

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separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

matrix active group

AQ-C18, polar modified bonding phase

particle size

2.7 μm

particle size

2.7 μm

particle size

2 μm

particle size

2.7 μm

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

product line

Ascentis®

pore size

90 Å pore size

pore size

90 Å pore size

pore size

90 Å pore size

pore size

90 Å pore size

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

Specificity

MAB1371Z is reactive with mouse ICAM-1 (CD54), which is present on T-cells, B-cells, monocytes and endothelial cells, epithelial cells, dendritic cells and keratinocytes. Expression of CD54 is increased after stimulation with cytokines and LPS, and it also plays a role in B cell-T cell interactions.

Application

Anti-ICAM-1 Antibody, clone 3E2B, Azide Free detects level of ICAM-1 & has been published & validated for use in ELISA, FC, FUNC, IC, IH.
Immunocytochemistry: Live or acetone fixed cells

ELISA

Immunohistochemistry of acetone fixed frozen sections. (Paraffin not tested).

Flow Cytometry: Suggested working dilution 50 μg/mL. Use 5 mL of this dilution to label 0.5 x 10(e6) cells.

Immunoprecipitation: use protein G or rabbit anti-hamster IgG secondary

Clone 3E2B is reported to be function blocking against murine ICAM-1.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Linkage

Replaces: CBL1331F

Physical form

Format: Purified
Purified IgG prepared by affinity chromatography on Protein G agarose beads. Liquid at 1 mg/mL in sterile-filtered PBS, containing no preservatives. Endotoxin level 4.13EU/mg

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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