Galactocerebroside (GalCer or GalC) is the most frequently found glycosphingolipid in animals. GalCer consist of a ceramide linked to a single galactose residue at the 1-hydroxyl moiety. Cerebrosides are concentrated in nervous tissues where they increase from about 0.02% of the dry weight in human fetal brain to 2% in gray matter and 12% in white matter of adult brain. GalCer is a specific cell-surface antigenic marker for oligodendrocytes and schwann cells. GalCer is the largest single component of the myelin sheath of nerves. GalCer synthesis therefore serves as a measurement of myelin formation or remyelination. Krabbe disease is an autosomal recessive sphingolipidosis caused by deficient activity of the lysosomal hydrolase galactosylceramide beta-galactosidase (GALC). GALC mediates the degradation of GalCer and other terminal beta-Gal-containing sphingolipids, including psychosine (galactosylsphingosine). Increased psychosine levels are believed to lead to widespread destruction of oligodendroglia in the CNS and to subsequent demyelination.

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically to the surface of oligodendrocytes and Schwann cells (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Target lipid structure is not species-specific.

Synaptic plasma membranes from bovine hippocampus (Ranscht, B. et al. (1982) Proc. Natl. Acad. Sci. U. S. A. 79(8):2709-2713).

Anti-Galactocerebroside, clone mGalC, Alexa Fluor^™ 647 conjugate, Cat. No. MAB342-AF647, is a highly specific mouse monoclonal antibody conjugate that targets galactocerebroside (GalC) and has been tested in Immunocytochemistry.

Research Category
Neuroscience

The unconjugated antibody (Cat. No. MAB342) and other conjugates (Cat. Nos. FCMAB312F, MAB342-AF555 and MAB342A4) are also available for ELISA, Flow Cytometry, Immunocytochemistry, and Immunofluorescence applications.

Evaluated by Immunocytochemistry in rat Schwann cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody immunostained non-permeabilzed rat schwann cells.

Protein A purified.

Purified mouse IgG3 Alexa Fluor^™ 647 conjugate in PBS with 1.5% BSA and 0.09% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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