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OP107

Sigma-Aldrich

Anti-BRCA1 (Ab-4) Mouse mAb (SD118)

liquid, clone SD118, Calbiochem®

Synonym(s):

Anti-Breast Cancer Susceptibility Protein

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250 MG
₩67,300
1 G
₩1,27,900

₩67,300


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250 MG
₩67,300
1 G
₩1,27,900

About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

₩67,300


Please contact Customer Service for Availability

Request a Bulk Order
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biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

SD118, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

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OP93OP16MABC199
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

Gene Information

human ... BRCA1(672)

Gene Information

human ... BRCA1(672)

Gene Information

human ... ERBB2(2064)

Gene Information

human ... BRCA1(672)

clone

SD118, monoclonal

clone

MS13, monoclonal

clone

7.16.4, monoclonal

clone

MS110, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

rat, human

species reactivity

human

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Anti-BRCA1 (Ab-4), mouse monoclonal, clone SD118, recognizes the ~220 kDa BRCA1 in HBL-100 and SK-BR3 cells. It is validated for WB, IF, IP, and for frozen sections.
Purified mouse monoclonal antibody generated by immunizing RBF/DnJ mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~220 kDa BRCA1 protein.
Recognizes the ~220 kDa BRCA1 protein in HBL-100 and SK-BR3 cells. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Immunogen

Human
a recombinant polypeptide corresponding to amino acids 1005-1313 in exon 11 of human BRCA1

Application

Frozen Sections (2 µg/ml)

Immunoblotting (2 µg/ml, see application references)

Immunofluorescence (2 µg/ml)

Immunoprecipitation (2 µg, see application references)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
HBL-100 or SK-BR3 cells

Other Notes

Chen, J.J., et al. 1999. Cancer Res.59, 1752s.
Cressman, V.L., et al. 1999. Cell Growth Differ.10, 1.
Irminger-Finger, I., et al. 1999. Biol. Chem.380, 117.
Xu, X., et al. 1999. Nat. Genet.22, 37.
Wilson, C.A., et al. 1999. Nat. Genet.21, 236.
Andres, J.L., et al. 1998. Oncogene16, 2229.
Chen, J., et al. 1998. Mol. Cell2, 317.
Chen, P.L., et al. 1998. Proc. Natl. Acad. Sci. USA95, 5287.
Fan, S., et al. 1998. Int. J. Cancer77, 600.
Gowen, L.C., et al. 1998. Science281, 1009.
Hsu, L.C. and R.L. White. 1998. Proc. Natl. Acad. Sci. USA95, 12983.
Shen, S.X., et al. 1998. Oncogene17, 3115.
Tulchin, N., et al. 1998. Int. J. Oncol.13, 513.
Ludwig, T., et al. 1997. Genes Dev11, 1226.
Ruffner, H. and I.M. Verma. 1997. Proc. Natl. Acad. Sci. USA94, 7138.
Scully, R., et al. 1997. Cell90, 425.
Thakur, S., et al. 1997. Mol. Cell Biol.17, 444.
Wilson, C.A., et al. 1997. Oncogene14, 1.
Cannon-Albright, L.A. and M.H. Skolnick. 1996. Semin Oncol.23, 1.
Chen, Y., et al. 1996. Cancer Res.56, 3168.
Liu, C.Y., et al. 1996. Genes Dev.10, 1835.
Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.
Scully, R., et al. 1996. Science272, 123.
Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.
Wilson, C.A. et al. 1996. Nat. Genet.13, 264.
Thompson, M.E., et al. 1995. Nat. Genet.9, 444.
Miki, Y., et al. 1994. Science266, 66.
Maximal BRCA1 levels are observed in subconfluent cell populations after serum stimulation. Highly specific BRCA1 antibody useful for blotting and IP of full length BRCA1 protein. Antibody should be titrated for optimal results in individual systems.

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Nikolaos Dimopoulos et al.
The Biochemical journal, 399(3), 473-481 (2006-07-11)
An increase in circulating levels of specific NEFAs (non-esterified fatty acids) has been implicated in the pathogenesis of insulin resistance and impaired glucose disposal in skeletal muscle. In particular, elevation of SFAs (saturated fatty acids), such as palmitate, has been
Nora Sandow et al.
Epilepsia, 50(4), 849-858 (2009-01-30)
Glutamine (GLN) is a precursor for synthesis of glutamate and gamma-aminobutyric acid (GABA) and has been found in the cerebrospinal fluid (CSF) at mean concentrations of 0.6 mM. Experiments on slices are usually performed in artificial CSF (aCSF) kept free
Differentiation of tumour and inflammation: characterisation of [methyl-3H]methionine (MET) and O-(2-[18F]fluoroethyl)-L-tyrosine (FET) uptake in human tumour and inflammatory cells
Barbara Stober et al.
European Journal of Nuclear Medicine, 33, 932-939 (2006)
J R Bading et al.
Nuclear medicine and biology, 23(6), 779-786 (1996-08-01)
The A system of amino acid transport is concentrative and thought to be a regulator of cell growth. The [11C]methyl alpha-aminoisobutyric acid (MeAIB) is prospectively an ideal tracer for transport measurements with PET, as it is not metabolized and concentrates
T Z Su et al.
Endocrinology, 139(3), 832-837 (1998-03-10)
System A is one of the most highly regulated transport systems for transport of neutral amino acids into mammalian cells. Stimulation of uptake of alpha-[3H]methylaminoisobutyric acid (MeAIB), a nonmetabolizable system A substrate, by a novel insulin-sensitizing agent, troglitazone, in 3T3-L1

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