11284932001

DNase I

from bovine pancreas

• Synonym(s): Dnase
• EC Number: 3.1.21.1 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine pancreas
• Quality Level: 100
• form: lyophilized
• packaging: pkg of 100 mg
• manufacturer/tradename: Roche
• technique(s): cell culture | mammalian: suitable
• storage temp.: 2-8°C

Description

General description

DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.

Application

DNase I has been used for tissue dissociation in conjunction with collagenases.

Biochem/physiol Actions

During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.

Features and Benefits

• Lyophilized
• Filtered through 0.2 μm pore size membrane before lyophilization
• Glycoprotein and double-strand-specific endonuclease

Unit Definition

One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.

Preparation Note

Activator: Bivalent metal ions (Ca²⁺, Mg²⁺)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg²⁺.

Reconstitution

Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash