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About This Item
Empirical Formula (Hill Notation):
C24H14Cl2O7
CAS Number:
Molecular Weight:
485.27
Beilstein:
68488
MDL number:
UNSPSC Code:
12352108
PubChem Substance ID:
NACRES:
NA.32
product line
BioReagent
Quality Level
Assay
≥95% (HPLC)
mp
232-234 °C (lit.)
solubility
DMSO: soluble
fluorescence
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
Related Categories
General description
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.
Application
2′,7′-Dichlorofluorescein diacetate is predominantly used to detect reactive oxygen (ROS) intermediates and quantify the overall oxidative stress in cells.
Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.
Dcfh-DA is used in the quantitative assay to measure oxidative stress in nanoparticle-treated cells.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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