D2532

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

• Synonym(s): blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution
• UNSPSC Code: 12161700
• NACRES: NA.25

Properties

• form: solution
• technique(s): Southern blotting: suitable
• foreign activity: DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
• storage temp.: −20°C

Description

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable