Quality Level
form
liquid
packaging
vial of 1.5 mL
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
−20°C
Application
10X PCR Buffer has been used as a component of the PCR mixture:
- for the amplification of viral DNA
- to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
- to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.
Features and Benefits
- Standalone buffer
- Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)
Preparation Note
Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin
Legal Information
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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