A8312

m-Aminophenylboronic acid–Agarose

aqueous suspension

• Synonym(s): (3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin
• MDL number: MFCD00801303
• UNSPSC Code: 12161501
• PubChem Substance ID: 329770955
• NACRES: NA.32

Properties

• form: aqueous suspension
• Quality Level: 300
• extent of labeling: 40-80 μmol per mL
• matrix: 6% beaded agarose
• matrix activation: epichlorohydrin
• matrix attachment: through amino to carboxyls of EDTA
• matrix spacer: 9 atoms
• capacity: ≥8 mg/mL binding capacity (peroxidase Type VI)
• storage temp.: 2-8°C
• SMILES string: [X]Nc1cc(ccc1)B(O)O

Description

General description

m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.

Application

m-Aminophenylboronic acid-Agarose has been used:

• as a component of the column for the purification of Escherichia coli TOP10F cells
• with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
• in the affinity purification of horseradish peroxidase (HRPO)
• in the glycated human serum albumin (gHSA) purification

Physical form

Suspension in water containing 0.002% chlorhexidine diacetate.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable