BCA1
Bicinchoninic Acid Kit for Protein Determination
for 200-1000 μg/ml protein
Synonym(s):
BCA protein assay
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About This Item
UNSPSC Code:
12352106
NACRES:
NA.32
Pricing and availability is not currently available.
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This Item | 706418 | C112852 | 376906 |
|---|---|---|---|
| assay 97% | assay 97% | assay 98% | assay 98% |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form powder | form powder | form liquid | form solid |
| mp 146-151 °C | mp 170-175 °C | mp 19 °C (lit.) | mp 122-124 °C (lit.) |
| functional group carboxylic acid | functional group carboxylic acid, hydroxyl, phenyl | functional group - | functional group carboxylic acid, hydroxyl, phenyl |
Application
Features and Benefits
- Simple, sensitive colorimetric assay for proteins
- Two stable reagents are used for the working solution
- Faster and easier than the Lowry protein assay
- Compatible with many detergents both ionic and nonionic
- Less variation between different proteins than the Bradford dye-binding assay
- Adaptable for use in microwell plates
Principle
Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.
Kit Components Only
Product No.
Description
- Bicinchoninic Acid Solution 1 L
- 4%(w/v) CuSO4 · 5H2O Solution 25 mL
- Protein Standard Solution 5 x 1
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Christiane Kiske et al.
Journal of agricultural and food chemistry, 67(4), 1187-1196 (2019-01-04)
The enantiomers of a homologous series (C6-C10) of 2-mercapto-4-alkanones were obtained by lipase-catalyzed kinetic resolution of the corresponding racemic 2-acetylthio-4-alkanones. Their configurations were assigned via vibrational circular dichroism and 1H NMR anisotropy based methods. Odor thresholds and odor qualities were
Chunze Li et al.
Chemical research in toxicology, 15(10), 1309-1317 (2002-10-22)
Chemically reactive species formed from the metabolism of carboxylic acid-containing compounds have been proposed as mediators of their toxic side-effects. Two alternative metabolic pathways known to be involved in the generation of reactive acylating metabolites of carboxylic acids are acyl
D Ahmad et al.
Chirality, 6(5), 365-371 (1994-01-01)
The significance of disturbances of lipid metabolism caused by xenobiotic acyl-CoAs as possible causes of peroxisomal proliferation has been studied with the enantiomers of 2-phenylpropionic acid (2-PPA), the (R)-enantiomer of which is converted to the acyl-CoA in rats while its
A J Hutt et al.
Chirality, 5(8), 596-601 (1993-01-01)
The metabolism of (R,S)-ibuprofen has been investigated in 24 microbial cultures. Of these Cunninghamella elegans, Mucor hiemalis, and Verticillium lecanii catalyzed the oxidation of the drug to 2-[4-(2-hydroxy-2-methylpropyl)phenyl]propionic acid, a known mammalian metabolite. The extent of metabolism was greatest with
D K Bhattacharyya et al.
The Journal of biological chemistry, 271(4), 2179-2184 (1996-01-26)
Examination of the crystal structure of the ovine prostaglandin endoperoxide synthase-1 (PGHS-1)/S- flurbiprofen complex (Picot, D., Loll, P.J., and Garavito, R.M. (1994) Nature 367, 243-2491) suggests (a) that the carboxyl group of arachidonic acid interacts with the arginino group of
Chromatograms
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