D5637

3,3′-Diaminobenzidine tetrahydrochloride hydrate

≥96%

• Synonym(s): (1,1′-Biphenyl)-3,3′,4,4′-tetramine tetrahydrochloride dihydrate
• Linear Formula: C₁₂H₁₄N₄ · 4HCl · xH₂O
• CAS Number: 868272-85-9
• EC Number: 231-018-9
• MDL number: MFCD08273058
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654109
• NACRES: NA.47

Properties

• Quality Level: 200
• Assay: ≥96%
• form: powder, crystals or chunks
• technique(s): titration: 96-104% (with NaOH)
• color: white to light brown, and Faint Red and Faint Grey to Light Grey
• mp: 280 °C
• solubility: H₂O: 50 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

Description

General description

3,3′-Diaminobenzidine tetrahydrochloride hydrate is a chromogen commonly used in immunohistochemistry and other staining techniques to visualize the presence of antigens or antibodies in biological samples. DAB HCl is designed as an immunohistology stain to visualize peroxidase activity in cells. DAB oxidation can be measured using a spectrophotometer.

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate is suitable for immunohistology staining procedures, western blotting, and FISH (fluorescence in situ hybridization). DAB is used in studying epithelial cell apoptosis in the event of early cataracts, and immunohistochemistry protocols of autosomal dominant polycystic kidney disease (PKD).

Biochem/physiol Actions

In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown-colored complex.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H302,H319,H341,H350
• Precautionary Statements: P202 - P264 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves