HT10132

Bouin′s solution

histological fixative

• Synonym(s): Bouin’s fluid
• MDL number: MFCD00146169
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• Quality Level: 200
• shelf life: Expiry date on the label
• composition: acetic acid, 5% ; formaldehyde, 9% ; picric acid, 0.9%
• color: yellow
• application(s): hematology; histology
• storage temp.: room temp

Description

General description

Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.

Application

Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-

• staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
• fixation of rat ovaries for the observing follicle cells using microscopy
• fixation of gonads of crab for morphological and histological analysis

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Biochem/physiol Actions

Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.

Safety Information

• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves