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Key Documents

M1544

Sigma-Aldrich

Resorufin methyl ether

Synonym(s):

7-Methoxy-3H-phenoxazin-3-one, Methoxyresorufin, O7-Methylresorufin

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About This Item

Empirical Formula (Hill Notation):
C13H9NO3
CAS Number:
Molecular Weight:
227.22
Beilstein:
209529
MDL number:
UNSPSC Code:
12161501
PubChem Substance ID:
NACRES:
NA.47

Assay

≥98.0% (HPLC)

Quality Level

form

powder

mp

≥220 °C (lit.)

solubility

dichloromethane: 0.95-1.05 mg/mL, clear, orange to very deep orange

storage temp.

2-8°C

SMILES string

COc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C13H9NO3/c1-16-9-3-5-11-13(7-9)17-12-6-8(15)2-4-10(12)14-11/h2-7H,1H3

InChI key

KNYYMGDYROYBRE-UHFFFAOYSA-N

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Application

Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).

Biochem/physiol Actions

Fluorimetric substrate for cytochrome P450 linked enzymes.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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