R7757

Red Blood Cell Lysing Buffer Hybri-Max^™

Liquid, sterile-filtered, suitable for hybridoma

• Synonym(s): RBC Lysing Buffer
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Red Blood Cell Lysing Buffer Hybri-Max^™, liquid, sterile-filtered, suitable for hybridoma
• sterility: sterile-filtered
• Quality Level: 400
• form: liquid
• technique(s): cell culture | hybridoma: suitable
• impurities: endotoxin, tested

Description

Application

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 × g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Biochem/physiol Actions

Red Blood Cell Lysing Buffer has been developed for use in hybridoma protocols to remove red blood cells from mouse splenocyte suspensions before fusion. It is also useful in systems where it may be desirable to remove red blood cells from cell suspensions, such as whole blood.

Preparation Note

Contains 8.3 g/L ammonium chloride in 0.01 M Tris-HCl buffer.

Other Notes

Note: This product is intended for the removal of red blood cells from mice. This product may not be appropriate for the lysis of red blood cells of other animals. The suitability of the product in any application other than mouse splenocytes must be determined by the researcher.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable