Diallyl trisulfide (DATS) is one of the stable naturally occurring bioactive polysulfide compounds found in Allium vegetables, especially in garlic.

Diallyl trisulfide (DATS) has been used as an exogenous hydrogen sulfide (H₂S)donor to study the effects on proliferation, apoptosis, and autophagy of human urothelial carcinoma (UC) cells in vitro.

Diallyl trisulfide elicits cardioprotective and anti-metabolic syndrome (MetS) effects against ex vivo induced myocardial ischemia/reperfusion (I/R) injury in MetS rats (male Wistar albino). It is a potent anti-cancer agent and acts by modulating different cancer-related pathways, inducing cell cycle arrest, apoptosis, metastasis, and angiogenesis in in vitro studies. DATS is also an effective therapeutic agent against multidrug resistance in triple-negative breast cancer (TNBC). DATS and its nanoemulsions display high antifungal activity against wood-rotting fungi such as T. hirsuta and L. sulphureus. It also provides protection against brain damage induced by ischemia/reperfusion (I/R) by activating nuclear factor erythroid 2-related factor 2 (Nrf2) in rat brains.