SRE0037

β-Glucuronidase from abalone

aqueous solution, β-glucuronidase ≥100,000 units/mL

• EC Number: 3.1.2.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: aqueous solution
• Quality Level: 200
• β-glucuronidase activity: ≥100,000 units/mL
• secondary activity: ≤8,000 units/mL sulfatase
• storage temp.: 2-8°C

Description

Application

Beta-glucuronidase from abalone has been used as a standard to evaluate its performance in benzodiazepine liquid chromatography-mass spectrometry (LC-MS/MS)confirmation assays.

Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine.

Biochem/physiol Actions

Beta-glucuronidase is anenzyme present in several animals, plants, and bacteria. It catalyzes thehydrolysis of Beta-glucuronide conjugates and plant glucuronides.Beta-glucuronidase is a member of the glycosyl hydrolases (GHs) family 2 and is ahomo-tetrameric glycoprotein. It facilitates the removal of glucuronic acidsfrom glucuronides and aids in the liver metabolism enzymes for the excretion ofendo- and xeno-biotics.

Unit Definition

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

Physical form

Supplied as an aqueous solution containing 0.85% NaCl and 0.02% sodium azide

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable