ABT15

Anti-ARHGEF6

from rabbit, purified by affinity chromatography

• Synonyme(s) : Rho guanine nucleotide exchange factor 6, Alpha-Pix, COOL-2, PAK-interacting exchange factor alpha, Rac/Cdc42 guanine nucleotide exchange factor 6
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_004831

Description

Description générale

Rho guanine nucleotide exchange factor 6 (UniProt Q15052; also known as Alpha-Pix, COOL-2, PAK-interacting exchange factor alpha, Rac/Cdc42 guanine nucleotide exchange factor 6) is encoded by the ARHGEF6 (also known as COOL2, KIAA0006, MRX46, PIXA) gene (Gene ID 9459) in human. ARHGEF6 (alpha-Pix) belongs to a family of guanine nucleotide exchange factors (GEF) that activate the Rho family of small GTPases. ARHGEF6 acts as a Cdc42 and Rac1 GEF and forms a signling module with the adaptor GIT (GPCR kinase interactor), the GTPase effector PAK (p21 activated kinase), and the GTPase activator PIX (PAK-interacting exchange factor), facilitating the activation Rac and Cdc42 GTPases at specific subcellular locations. In contrast to the ubiquitious expression pattern of ARHGEF7 (beta-Pix), ARHGEF6 is only detected in highly motile cells, including immune cells, microvascular cells, and growing fetal neurons, where it plays an essential role in mediating cell spreading, directional migration, angiogenesis, and fetal neuron dendrite outgrowth. In addition, both ARHGEF6 and ARHGEF7 are shown to interact with the RING type E3 ubiquitin ligase c-Cbl. While ARHGEF6 is a substrate for c-Cbl-mediated proteasomal degradation, ARHGEF7 binds and antagonizes against c-Cbl-mediated EGFR proteasomal degradation. Elevated ARHGEF6 accumulation is found in human A172 and rat C6 glioma cells that do not express c-Cbl, resulting in enhanced cell migration and invasion.

Spécificité

This polyclonal antibody targets an internal sequence C-terminal to the PH domain.

Immunogène

KLH-conjugated linear peptide corresponding to an internal sequence C-terminal to the PH domain.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a represenative lot detected ARHGEF6 in A431, HeLa, and NIH/3T3 cells.

Western Blotting Analysis: A 1:500 dilution from a represenative lot detected ARHGEF6 in 10 µg of human placenta tissue lysate as well as in 10 µg of A431, HeLa, HepG2, HUVEC, Jurkat, and PC3 cell lysates.

Research Category
Cell Structure

This rabbit polyclonal Anti-ARHGEF6 Antibody, Cat. No. ABT15 is validated for use in Immunocytochemistry and Western Blotting for the detection of Rho guanine nucleotide exchange factor 6.

Qualité

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGEF6 in 10 µg of HEK293 cell lysate.

Description de la cible

~71 kDa observed. 87.50/70.79 kDa (human isoform 1/2), 87.05 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable