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MABS194

Sigma-Aldrich

Anti-c-K-Ras Antibody, clone 234-4.2

clone 234-4.2, from mouse

Synonyme(s) :

GTPase Kras, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras

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100 MG
CA$71.90

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

CA$71.90


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Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

234-4.2, monoclonal

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

rat (immunogen homology)

Technique(s)

immunohistochemistry: suitable
western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

Description générale

GTPase KRas (c-k-ras; K-ras) is a membrane-bound G protein belonging to the family of Ras GTPases. They are activated by the binding of GTP in response to signals from various receptors including the epidermal growth factor receptor (EGFR). Ras GTPases are regulated by guanine nucleotide exchange factors (GEF) and GTPase activating proteins (GAP) and lie upstream of the MAP kinase pathway which controls a range of cellular processes including proliferation and differentiation. Previous studies have suggested that KRas plays an important role in embryonic development, and it may also contribute to the pathogenesis of colorectal cancer via an EGFR-mediated pathway.

Immunogène

Recombinant protein corresponding to rat c-K-Ras.

Application

Research Category
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-c-K-Ras Antibody, clone 234-4.2 is validated for use in Western Blotting, IHC for the detection of c-K-Ras.
Western Blot Analysis: A representative lot detected c-K-Ras in A431 cell lysate.

Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).

Qualité

Evaluated by Western Blot in SW-480 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.

Description de la cible

~22 kDa observed

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
SW-480 cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Haisu Shi et al.
Microbial cell factories, 15(1), 117-117 (2016-07-02)
Delta-6 desaturase (FADS6) is a key bifunctional enzyme desaturating linoleic acid (LA) or α-linolenic acid (ALA) in the biosynthesis of polyunsaturated fatty acids (PUFAs). In previous work, we analyzed the substrate specificity of two FADS6 enzymes from Mortierella alpina ATCC
Yixuan Fan et al.
Meat science, 157, 107872-107872 (2019-07-06)
This study aims to investigate the effect of algae supplementation in high-energy diet (HE diet) on lipid metabolism of intensive feeding sheep. The lambs were assigned to two groups and received a standard diet (ST diet, 8.40 MJ/kg) or a HE
Effect of microwave cooking or broiling on selected nutrient contents, fatty acid patterns and true retention values in separable lean from lamb rib-loins, with emphasis on conjugated linoleic acid
Maranesi.M, et al.
Food Chemistry, 90, 207-218 (2005)
Ronan Lordan et al.
Foods (Basel, Switzerland), 8(5) (2019-05-30)
The in vitro antithrombotic properties of polar lipid constituents of malted grain (MG), pelleted hops (PH), brewer's spent grain (BSG), spent hops (SH), wort, and bottled beer from the same production line were assessed in human platelets. The total lipids
Effect of microwave cooking or broiling on selected nutrient contents, fatty acid patterns and true retention values in separable lean from lamb rib-loins, with emphasis on conjugated linoleic acid
Maranesi.M, et al.
Food Chemistry, 90(1-2), 207-218 (2005)

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