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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
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Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

P3C9, monoclonal

Espèces réactives

rat, mouse

Réactivité de l'espèce (prédite par homologie)

rhesus macaque (based on 100% sequence homology), porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology), rabbit (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology)

Technique(s)

western blot: suitable

Isotype

IgG2bκ

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

ambient

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

bovine ... Gjc1(511119)
dog ... Gjc1(490936)
human ... GJC1(10052)
mouse ... Gjc1(14615)
pig ... Gjc1(100048959)
rat ... Gjc1(266706)
rhesus monkey ... Gjc1(715463)

Description générale

Gap junction gamma-1 protein (UniProt: A4GG66; also known as Connexin-45, Cx45, Gap junction alpha-7 protein) is encoded by the Gjc1 (also known as Gja7) gene (Gene ID: 266706) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-45 is a multi-pass membrane protein with ubiquitous expression. It consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low molecular weights diffuse from one cell to a neighboring cell. Connexin 45 is the first cardiac connexin expressed during embryonic development and plays an important role in propagating the action potential from the conduction system to the working myocardium. Connexin 45 gap junction channels are reported to close when the membrane potential becomes negative. This prevents retrograde conduction from myocardium to the conduction system.

Spécificité

Clone clone P3C9 detects Connexin-45 in rat and mouse cells. It targets an epitope within 14 amino acids from the C-terminal half of rat connexin 45.

Immunogène

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of rat connexin 45.

Application

Research Category
Cell Structure
This mouse monoclonal Anti-Connexin 45, clone P3C9, Cat. No. MABT900 is tested for use in Western Blotting for the detection of Connexin-45.
Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Manias, J.L., et. al. (2008). Cardiovasc Res. 80(3):385-95).

Western Blotting Analysis: A representative lot detected Connexin-45 in Western Blotting applications (Kopanic, J.L., et. al. (2013). Front Pharmacol. 4:106).

Qualité

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Connexin 45 in 10 µg of mouse heart tissue lysate.

Description de la cible

~45 kDa observed; 45.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1


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