Peptone from pancreatic digest of casein is used as a nitrogen source in culture media formulated for isolating and cultivating fastidious and non-fastidious bacteria, fungi and some protozoa. It is a refined hydrolysate with high solubility and is used as a microbiological nutrient. It is a rich source of amino acids and peptides recommended for culture media in analytical microbiology and in industrial fermentation.

Peptone from casein, a pancreatic digest is recommended for laboratory media and fermentation and has been used in antibiotics, toxins, and enzyme productions.