F0888

Fura 2-AM

≥95% purity (HPLC), powder

• Synonyme(s) : Fura-2 pentakis(acetoxymethyl) ester
• Formule empirique (notation de Hill) : C₄₄H₄₇N₃O₂₄
• CAS Number: 108964-32-5
• Poids moléculaire : 1001.85
• Beilstein: 8183748
• Numéro MDL: MFCD00036976
• Code UNSPSC : 12171500
• ID de substance PubChem : 24894734
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Fura 2-AM, ≥95% (HPLC)
• Niveau de qualité: 100
• Essai: ≥95% (HPLC)
• Forme: powder
• Couleur: yellow to yellow-green
• Solubilité: acetone: 10 mg/mL
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: −20°C
• Chaîne SMILES: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

Description

Description générale

Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505nm, and the excitation peak ranges between 340-380nm and is the preferred calcium indicator.

Application

Fura 2-AM is a popular probe used to determine dynamic changes in cytosolic free calcium in intact living cells.

Caractéristiques et avantages

The major benefits of using the ester form of Fura 2 as Fura 2-AM are: It is cell-permeable. It can be loaded into cells easily. Another advantage of using Fura 2-AM over single wavelength dyes is that the ratio signal is independent of : The dye concentration. Illumination intensity, and Optical path length.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)