94403

Hoechst 33258 solution

1 mg/mL in H₂O, ≥98.0% (HPLC)

• Synonyme(s) : bisBenzimide H 33258 solution
• Formule empirique (notation de Hill) : C₂₅H₂₄N₆O · 3HCl
• CAS Number: 23491-45-4
• Poids moléculaire : 533.88
• Numéro MDL: MFCD29074011
• Code UNSPSC : 12171500
• ID de substance PubChem : 329770334
• Nomenclature NACRES : NA.32

Propriétés

• Niveau de qualité: 100
• Essai: ≥98.0% (HPLC)
• Forme: liquid
• Concentration: 1 mg/mL in H₂O
• Solubilité: DMF: soluble; H₂O: soluble
• Fluorescence: λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• Température de stockage: 2-8°C
• Chaîne SMILES: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
• Clé InChI: SMNPLAKEGAEPJD-UHFFFAOYSA-N

Description

Description générale

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable