A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

• Synonyme(s) : ACP, Lysyl endopeptidase
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bacterial (LYSOBACTER SP. BACTERIA M497-1)
• Niveau de qualité: 200
• Forme: lyophilized powder
• Activité spécifique: ≥1,000 units/mg solid
• Poids mol.: 27 kDa
• Technique(s): cell based assay: suitable
• Adéquation: suitable for cell lysis
• Application(s): diagnostic assay manufacturing
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Actions biochimiques/physiologiques

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀=0.6 over 2 hours at 37 °C.

Définition de l'unité

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Forme physique

crude powder

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P342 + P311
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves