C3172

Creatininase from microorganisms

lyophilized powder, 100-300 units/mg protein

• Synonyme(s) : Creatinine Amidohydrolase
• CAS Number: 9025-13-2
• Numéro CE : 3.5.2.10 (BRENDA, IUBMB)
• Numéro CE : 232-786-8
• Numéro MDL: MFCD00130877
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: lyophilized powder
• Niveau de qualité: 200
• Activité spécifique: 100-300 units/mg protein
• Poids mol.: ~175 kDa
• Composition: Protein, 65-85%
• Activité étrangère: Creatinase and urease ≤1%; Hexokinase and ATPase ≤0.1%
• Température de stockage: 2-8°C

Description

Application

Creatininase from microorganisms may be used in the preparation of amperometric biosensor by co-immobilization with other enzymes for the determination of creatinine.

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Actions biochimiques/physiologiques

Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.

Propriétés physiques

Isoelectric point:4.7
Michaelis constants:3.2 x 10‾²M (Creatinine), 5.7 x 10‾²M (Creatine)
Structure:6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Inhibitors:Ag⁺, Hg⁺⁺, N-bromosuccinimide, EDTA
Optimum pH:6.5 − 7.5
Optimum temp:70°C
pH Stability:pH 7.5 − 9.0 (5°C, 16hr)
Thermal stability:Below 70°C (pH 7.5, 30 min)

Définition de l'unité

One unit will hydrolyze 1.0 μmole of creatinine to creatine per min at pH 8.0 and 25 °C

Forme physique

Lyophilized powder containing sucrose and BSA as stabilizers

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)