D2197

Diaphorase from Clostridium kluyveri

recombinant, expressed in E. coli, Animal-component free

• Synonyme(s) : Diaphorase, Lipoamide Dehydrogenase, Lipoyl Dehydrogenase
• Numéro CE : 1.8.1.4 (BRENDA, IUBMB)
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 200
• Forme: solid
• Activité spécifique: >=30 units/mg protein (biuret)
• Température de stockage: −20°C

Description

Description générale

Research area: Cancer

Application

Diaphorase from Clostridium kluyveri has been used:

• in lactate dehydrogenase assay in gill cells.
• impregnated on the strip for formate dehydrogenase assay.
• in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
• for semiquinone generation during benzoquinone reduction.
• for evaluation of glucose uptake in cultured muscle cells.
• in lactate dehydrogenase cytotoxicity assay.

Actions biochimiques/physiologiques

Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.

Définition de l'unité

One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.

Forme physique

Supplied as a lyophilized powder containing trehalose.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable