L5418

Lipopolysaccharides from Escherichia coli O55:B5

Ready Made solution, 1 mg/mL

• Synonyme(s) : LPS
• CAS Number: 93572-42-0
• Numéro MDL: MFCD00164401
• Code UNSPSC : 12352201
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: Escherichia coli (O55:B5)
• Niveau de qualité: 200
• Forme: aqueous solution
• Concentration: 1 mg/mL
• Couleur: white to faint yellow
• Solubilité: water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O55:B5. The source strain is CDC 1644-70. This LPS serotype has been used in studies of septic shock and to induce myocarditis.

Application

Les lipopolysaccharides (LPS) sont des constituants caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné via le récepteur TLR4 (Toll-Like Receptor 4), un membre de la famille des récepteurs de type Toll, qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)