M1255

Calcein Blue

Powder

• Synonyme(s) : 4-Methylumbelliferone-8-methyliminodiacetic acid
• Formule empirique (notation de Hill) : C₁₅H₁₅NO₇
• CAS Number: 54375-47-2
• Poids moléculaire : 321.28
• Numéro CE : 259-129-8
• Numéro MDL: MFCD00006867
• Code UNSPSC : 12171500
• ID de substance PubChem : 24896629
• Nomenclature NACRES : NA.47

Propriétés

• Product Name: Calcein Blue, fluorescent dye
• Niveau de qualité: 200
• Forme: powder
• Technique(s): titration: suitable
• Couleur: white to light yellow
• Solubilité: 1 M NaOH: 50 mg/mL
• Fluorescence: λ_ex 322 nm; λ_em 445 nm in methanol
• Adéquation: suitable for indicator for EDTA titration
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12
• InChI: 1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
• Clé InChI: RJPSHDMGSVVHFA-UHFFFAOYSA-N

Description

Description générale

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Application

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H315,H319,H335
• Conseils de prudence: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves