M2003

MTT Formazan

powder

• Synonyme(s) : Thiazolyl blue formazan
• Formule empirique (notation de Hill) : C₁₈H₁₇N₅S
• CAS Number: 57360-69-7
• Poids moléculaire : 335.43
• Numéro MDL: MFCD00080731
• Code UNSPSC : 12171500
• ID de substance PubChem : 24896591
• Nomenclature NACRES : NA.47

Propriétés

• Essai: ≥97% (TLC)
• Niveau de qualité: 200
• Forme: powder
• Couleur: purple
• Solubilité: acetone: 1 mg/mL
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• Clé InChI: GIFKVQOPAJDJLO-SZVLODPRSA-N

Description

Application

MTT Formazan has been used to measure cell viability.

Actions biochimiques/physiologiques

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)