NA0400

GenElute^™ HP Endotoxin-Free Plasmid Maxiprep Kit

sufficient for 10 preparations

• Synonyme(s) : Gen Elute, GenElute Endotoxin-Free Plasmid Kit
• Code UNSPSC : 41105501
• Nomenclature NACRES : NA.52

Propriétés

• Utilisation: sufficient for 10 preparations
• Niveau de qualité: 200
• Caractéristiques du produit alternatif plus écologique: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• Technique(s): DNA extraction: suitable
• Autre catégorie plus écologique: Aligned,
• Température de stockage: 15-25°C

Description

Description générale

The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Caractéristiques et avantages

• Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
• Only 35 minutes from pelleted cells to purified plasmid
• Convenient vacuum format

Principe

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.

Autres remarques

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informations légales

GenElute is a trademark of Sigma-Aldrich Co. LLC

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H290,H302,H315,H319,H334,H336
• Conseils de prudence: P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Classification des risques: Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Central nervous system
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable