Y1771

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

• Synonyme(s) : Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
• Numéro MDL: MFCD01095679
• Code UNSPSC : 41106212
• Nomenclature NACRES : NA.85

Propriétés

• Niveau de qualité: 200
• Forme: powder
• Technique(s): transformation: suitable
• Application(s): microbiology
• Température de stockage: room temp

Description

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

• the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
• Yarrowia lipolytica PO1f strain positive transformants
• CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Notes préparatoires

Dissolve 1.46 g of supplement per liter of medium.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable