Y2146

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

• Synonyme(s) : Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements
• Numéro MDL: MFCD01095679
• Code UNSPSC : 41106212
• Nomenclature NACRES : NA.85

Propriétés

• Niveau de qualité: 200
• Forme: powder
• Technique(s): transformation: suitable
• Application(s): microbiology
• Température de stockage: room temp

Description

Application

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

Autres remarques

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Notes préparatoires

Dissolve 1.46 g of supplement per liter of medium.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable