B6185
Bionic™ Buffer, 10X Concentrate
for Northern and Southern blotting, solution
Synonym(s):
Sodium Borate Electrophoresis Buffer
grade
Molecular Biology
Quality Level
description
Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.
form
solution
concentration
10 ×
impurities
DNase, RNase and nickase, none detected
pH
8.0-8.5 (20-25 °C)
storage temp.
room temp
General description
Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.
Application
Suitable for:
- Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
- Use with TBA and TAE gels
- Preparation of agarose gels
Features and Benefits
- Faster run times
- Sharper bands
- Easy to dilute and use
- Flexible use with a variety of gel types
Packaging
The 4 L and 10 L sizes are supplied in dispensers with a spigot.
Caution
Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.
Legal Information
Bionic is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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