废水和生产用水检测
废水和生产用水处理是保护公共卫生与环境并保持工业生产效率的一道重要屏障。大多数监管机构都要求定期分析检测各处理环节的废水排放情况。此外，通常还要求分析锅炉和冷却器系统中生产用水的化学成分。
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NGS方法概览
随着NGS方法和试剂不断发展，目前涌现出众多可供研究人员选用的NGS系统。现有常用平台整合了NGS流程中的多个关键步骤，包括样品或文库制备、簇生成、测序和数据分析。样品制备通常涉及DNA扩增或添加序列接头。DNA序列簇生成指DNA共价连接接头后，与固体表面杂交进行桥式PCR扩增，或者进行微乳液PCR。现有多种DNA测序方法，包括连接法测序、合成法测序、焦磷酸测序和离子半导体测序。每种测序方法都采用不同的反应步骤和化学法，最终决定序列长度（读长）、错误率和试剂成本。
NGS数据分析方法
测序后的数据分析是所有NGS工作流程最后的关键步骤。由于每个NGS平台和工作流程都会生成海量数据信息，生物信息学家必须借助种类繁多的分析工具（如Bowtie、Galaxy等）来分析这些原始数据，进行序列比对和定位。如此庞大数据集需要通过融合各个学科来开发和优化各类分析和解读工具，这也是推动NGS技术领域不断发展的重要动力。根据特定的应用需求，这些强大的工具可帮助研究人员测序整个基因组、外显子组或转录组来进行基础和疾病研究。
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