DNA和RNA纯化

推荐类别
DNA和RNA样品往往从“粗制”样品中获得。基因组DNA、质粒DNA和总RNA可以从细菌和哺乳动物细胞、植物组织、真菌组织、哺乳动物组织、血液、血浆、血清、病毒、口腔和鼻拭子、凝胶基质、PCR及其他酶促反应等多种来源中提取和纯化。上述样品经细胞膜裂解或样品均质化,再对蛋白质、酶、去污剂、盐和脂质进行去除,即可分离出核酸。
常见方法包括:
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最终用途决定了使用何种纯化方法。下游的应用包括PCR、qPCR、限制性酶切、连接、克隆、基因分型、基因表达分析、下一代测序(NGS)以及DNA和RNA印迹等。
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