Produktinformationen zu kundenspezifischen DNA- und RNA-Oligos & qPCR-Sonden

Seit über 35 Jahren bieten wir kundenspezifische und vordefinierte Oligos und qPCR-Sonden für PCR, Klonierung, DNA-Sequenzierung und Genexpression an. 

Wir bieten Tools für das Oligo- und qPCR-Sondendesign an, darunter OligoArchitect™ Primer and Sondendesign-Lösungen und OligoEvaluator™: Oligonukleotid-Sequenz-Rechner, der Primer-Analysewerte für die PCR liefert und bei der Bestimmung der Volumina für die Resuspension und Verdünnung Ihrer DNA-Oligos auf die gewünschten Endkonzentrationen unterstützt.

So bestellen Sie Oligo-Produkte online

Tutorial zum Bestellen von kundenspezifischen Oligo-Produkten. Hervorhebung der Navigation zur Bestellung, manuellen Eingabe und Mehrfacheingabe von DNA-Oligos, DNA in Platten, qPCR-Sonden, siRNA und vordefinierter siRNA.

Empfohlene Artikel und Protokolle

• OligoEvaluator™ for T_m Calculation & Primer Analysis
  We offer OligoEvaluator™ as an easy-to-use Oligo Analyzer tool for online oligonucleotide sequence calculator that provides primer dimer analysis values for PCR and calculation of T_m.
• DNA Oligonucleotide Synthesis
  Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
• Annealing Oligonucleotides Protocol
  Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.

Technische Literatur

• DNA Oligonucleotide Synthesis
  Learn about the steps in phosphoramidite solid-phase method of Oligonucleotide Synthesis.
• Oligonucleotide Purification
  Discover the best purification option for your oligo, based on the intended application.
• Oligonucleotide Quantification
  DNA can be easily quantitated in a UV spectrometer due to its highly conjugated nature.
• Phosphorothioate Oligonucleotides
  Phosphorothioate is a widely used backbone variant effective in creating oligonucleotides for antisense knockdowns.
• Phosphorothioate Chromatography Profile
  The ultimate goal of most antisense research is to develop therapeutic agents for diseases such as diabetes, cancer, and HIV/ AIDS. Phosphorothioate oligonucleotides (commonly referred to as S-oligos) are often the molecules of choice because of their resistance to cellular nuclease degradation.
• Choosing the Right Custom qPCR Probe
  View a summary of the applications using commonly ordered custom qPCR probes.
• Dual-Labeled Probes in Digital PCR
  Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
• Locked Nucleic Acid
  Frequently asked questions about Locked Nucleic Acids (LNA)
• BLACK HOLE QUENCHER™ (BHQ™)
  Two commonly used quenchers, TAMRA™ and DABYCL, limit the ultimate sensitivity and flexibility of qPCR.
• Oligonucleotide Handling & Stability
  Handing and stability techniques to reduce variation and ensure trouble-free experiments.
• Oligonucleotide Melting Temperature
  Learn about methods for calculating oligonucleotide melting temperature (Tm).

Protokolle, Verfahren und Qualität

• Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
  Protocol for conjugating NHS-ester modifications to an oligonucleotide with an amino label.
• Annealing Oligonucleotides Protocol
  Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences.
• Oligo Reduction
  Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides.
• Oligonucleotide Quality Control & Quality Assurance
  The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
• ISO-Zertifikate und qualitätsbezogene Standort-Selbstbewertungen
  Wir verpflichten uns zu Produktqualität und Produktionseffizienz. Es ist unser erklärtes Ziel, immer die Erwartungen unserer Kunden zu erfüllen.

Videos

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Compliant Oligo Manufacturing

Video overview of our capabilities in oligo scale-up and manufacturing

Produktzitationen & Unsere Publikationen

1. Guo Y, Ye JY, Divin C, Huang B, Thomas TP, Baker, Jr. JR, Norris TB. 2010. Real-Time Biomolecular Binding Detection Using a Sensitive Photonic Crystal Biosensor. Anal. Chem.. 82(12):5211-5218. https://doi.org/10.1021/ac100576y

2. Kudla G, Murray AW, Tollervey D, Plotkin JB. 2009. Coding-Sequence Determinants of Gene Expression in Escherichia coli. Science. 324(5924):255-258. https://doi.org/10.1126/science.1170160

3. Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F. Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation. PLoS ONE. 4(12):e8291. https://doi.org/10.1371/journal.pone.0008291

4. Omumi A, Beach DG, Baker M, Gabryelski W, Manderville RA. 2011. Postsynthetic Guanine Arylation of DNA by Suzuki?Miyaura Cross-Coupling. J. Am. Chem. Soc.. 133(1):42-50. https://doi.org/10.1021/ja106158b

5. Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel SS. 2009. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 462(7275):940-943. https://doi.org/10.1038/nature08611

6. Wang Z, Elbaz J, Remacle F, Levine RD, Willner I. 2010. All-DNA finite-state automata with finite memory. Proceedings of the National Academy of Sciences. 107(51):21996-22001. https://doi.org/10.1073/pnas.1015858107

7. Yang L, Kemadjou JR, Zinsmeister C, Bauer M, Legradi J, Müller F, Pankratz M, Jäkel J, Strähle U. 2007. Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo. Genome Biol. 8(10):R227. https://doi.org/10.1186/gb-2007-8-10-r227

8. Zykovich A, Korf I, Segal DJ. 2009. Bind-n-Seq: high-throughput analysis of in vitro protein?DNA interactions using massively parallel sequencing. 37(22):e151-e151. https://doi.org/10.1093/nar/gkp802

9. Okabe K, Harada Y, Zhang J, Tadakuma H, Tani T, Funatsu T. 2011. Real time monitoring of endogenous cytoplasmic mRNA using linear antisense 2?-O-methyl RNA probes in living cells. 39(4):e20-e20. https://doi.org/10.1093/nar/gkq1196