CS0760

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

• Número CE: 217-896-6
• Código UNSPSC: 12352207
• NACRES: NA.32

Propiedades

• Nivel de calidad: 200
• uso: kit sufficient for 1000 reactions (using 96 multiwell plates); kit sufficient for 50 reactions (in a 2 mL cuvette)
• envase: pkg of 1 kit
• condiciones de almacenamiento: dry at room temperature
• técnicas: flow cytometry: suitable; protein staining: suitable
• fluorescencia: λ_ex 490 nm; λ_em 590 nm (JC-1 dye)
• aplicaciones: cell analysis; detection
• método de detección: fluorometric
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Aplicación

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Características y beneficios

• Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
• Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
• Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principio

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Nota de análisis

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Solo componentes del kit

• DMSO 1 mL
• JC-1 Assay Buffer 5X 25 mL
• JC-1 Stain 25 μg
• Valinomycin Ready Made 100 μL

Información de seguridad

• Pictogramas: GHS06
• Palabra de señalización: Danger
• Frases de peligro: H300 + H310
• Consejos de prudencia: P262 - P264 - P280 - P301 + P310 - P302 + P352 + P310 - P361 + P364
• Clasificaciones de peligro: Acute Tox. 1 Dermal - Acute Tox. 1 Oral
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable