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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
monoclonal
species reactivity
bovine
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr495)
Gene Information
mouse ... Nos3(287024)
Specificity
Predicted cross-reactivity with human, rabbit, mouse and rat
Recognizes phosphorylated eNOS/NOS III.
Immunogen
Peptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
Application
This Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Western Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Quality
routinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Target description
135 kDa
Linkage
Replaces: 05-811
Physical form
Cultured supernatant in 0.05% sodium azide
Analysis Note
Control
Fetal bovine heart endothelial cell lysate
Fetal bovine heart endothelial cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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