Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies.

Detects SMAD1

Other species were not tested.

GST-fusion protein corresponding to amino acids 1-110 of human SMAD1.

Anti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF.

Research Category
Signaling

Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.

Routinely evaluated by Western Blot

Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.

52 kDa

Replaces: 04-1100

Format: Purified

Protein G Purified

Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3.

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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