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05-1471

Sigma-Aldrich

Anti-Rictor Antibody, clone 9F1.2

clone 9F1.2, from mouse

Synonym(s):

Rictor, AVO3, rapamycin-insensitive companion of mTOR, mAVO3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.
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biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9F1.2, monoclonal

species reactivity

human, mouse, rat

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_689969.2

UniProt accession no.

Q6R327

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manufacturer/tradename

Goodfellow 435-987-84

manufacturer/tradename

Goodfellow 655-154-51

manufacturer/tradename

Goodfellow 595-052-73

manufacturer/tradename

Goodfellow 682-680-00

form

rod

form

rod

form

rod

form

rod

assay

99.95%

assay

99.95%

assay

99.95%

assay

99.95%

mp

1083.4 °C (lit.)

mp

1083.4 °C (lit.)

mp

1083.4 °C (lit.)

mp

1083.4 °C (lit.)

resistivity

1.673 μΩ-cm, 20°C

resistivity

1.673 μΩ-cm, 20°C

resistivity

1.673 μΩ-cm, 20°C

resistivity

1.673 μΩ-cm, 20°C

bp

2567 °C (lit.)

bp

2567 °C (lit.)

bp

2567 °C (lit.)

bp

2567 °C (lit.)

General description

Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.

Specificity

This antibody recognizes Rictor.

Immunogen

Recombinant protein corresponding to human Rictor.

Application

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.
Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.

Quality

Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.

Target description

190 kDa Observed

Physical form

Format: Purified
Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Analysis Note

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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