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1.16450

Sigma-Aldrich

AFB-Color staining kit

for the microscopic investigation of acid-fast bacteria (cold staining)

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100 μL
MYR 1,403.60

MYR 1,403.60


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100 μL
MYR 1,403.60

About This Item

UNSPSC Code:
41116130
NACRES:
NA.74

MYR 1,403.60


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Quality Level

IVD

for in vitro diagnostic use

application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

storage temp.

15-25°C

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This Item
BAF175750003BAF175750040BAF175750020
sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

binding type

non-treated surface

binding type

non-treated surface

binding type

non-treated surface

binding type

non-treated surface

material

colorless tube, polyethylene cap, white , flat bottom, polystyrene tube

material

colorless tube, flat bottom, polyethylene cap, polystyrene tube, white

material

colorless tube, flat bottom, polyethylene cap, polystyrene tube, white

material

colorless tube, flat bottom, polyethylene cap, polystyrene tube, white

capacity

111 mL

capacity

11 mL

capacity

148 mL

capacity

74 mL

manufacturer/tradename

Bel-Art F17575-0030

manufacturer/tradename

Bel-Art F17575-0003

manufacturer/tradename

Bel-Art F17575-0040

manufacturer/tradename

Bel-Art F17575-0020

packaging

pack of 72 ea

packaging

pack of 144 ea

packaging

pack of 72 ea

packaging

pack of 144 ea

General description

The AFB-Color staining kit - for the microscopic investigation of acid-fast bacteria (AFB) (cold staining), is a ready-to-use solution used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures. The AFB-Color staining set is based on the Ziehl-Neelsen staining method for the detection of acid-fast bacteria. The set is modified such, that heating of the carbolfuchsin solution is dispensable. Consequently, the release of potentially hazardous phenolic vapors will be avoided.The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. The AFB-Color Kit offers a modified AFB-Color Carbol fuchsin solution which abolishes heating (Kinyoun method, cold staining).Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye.
Pretreatment of the specimens with Sputofluol dissolves the bacteria from the surrounding viscid sputum and cell material. The package is sufficient for up to 250 applications.This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Suitability for microscopy: passes test
Mycobacteria: red
Background: green

Legal Information

SPUTOFLUOL is a trademark of Merck KGaA, Darmstadt, Germany

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1A

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

WGK

WGK 3


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