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176908

Sigma-Aldrich

Anthrax Protective Antigen, Bacillus anthracis, Nicked, Recombinant, B. anthracis

Synonym(s):

PA 63, PA 63

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77
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form

lyophilized

Quality Level

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

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This Item
124036-M676496344092
assay

≥95% (HPLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

assay

≥98% (sum of two geometrical isomers, HPLC)

form

lyophilized solid

form

solid

form

lyophilized solid

form

solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

DMSO: 1 mg/mL, acetic acid: 5%

solubility

DMSO: 25 mg/mL

solubility

DMSO: 5 mg/mL, water: soluble

solubility

DMSO: 50 mg/mL

General description

Recombinant, Bacillus anthracis anthrax protective antigen (nicked) expressed in a specialized strain of B. anthracis. PA63 is the carboxyl-terminal 63-kDa fragment obtained by the proteolytic cleavage of the receptor-bound protective antigen (PA), one of the three proteins that comprise anthrax toxin. Unlike native PA, PA63 oligomerizes to form a ring-shaped heptamer that binds up to three copies of EF and/or LF competitively and with high affinity (Kd ~1 nM). Whereas native PA persists on the cell surface, the heptamer is endocytosed, presumably because oligomerization aggregates anthrax toxin receptor. The endocytosed toxic complexes are trafficked to endosomal compartments where the low pH causes the PA63 heptamer to insert into the membrane and form a water-filled channel.

Biochem/physiol Actions

Cell permeable: no
Primary Target
EF and/or LF
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM NaCL, 10 mM BIS-Tris propane, 1.25% trehalose, pH 8.5.

Reconstitution

Following reconstitution aliquot and freeze (-20°C or- 70°C) for long term storage or refrigerate (4°C) for short-term (few hours) storage. Avoid rapid freeze-thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C.
Reconstitute in sterile 50% glycerol or sterile, distilled H₂O to a final concentration of 1 mg/ml. Addition of BSA to a concentration of 1 mg/ml may enhance stability.

Analysis Note

Single major band at 63 kD by SDS-PAGE

Other Notes

Mogridge, J., et al. 2002. Proc. Natl. Acad. Sci. USA99, 7045.
Nassi, S., et al. 2002. Biochemistry41, 1445.
Singh, Y., et al. 1999. Infect. Immun.67, 1853.
Leppla, S.H. 1988. Methods Enzymol.165, 103.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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