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347420

Sigma-Aldrich

β-Glucuronidase, Helix pomatia

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

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About This Item

EC Number:
UNSPSC Code:
12352202
NACRES:
NA.54
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biological source

Helix pomatia

Quality Level

description

Merck USA index - 14, 4466

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

solubility

aqueous buffer: soluble

foreign activity

Aryl sulfatase ≤2%

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.
Native β-glucuronidase from Helix pomatia. Useful for hydrolyzing urinary steroid conjugates prior to steriod assays. Note: 1 MU = 1,000,000 units.

Biochem/physiol Actions

β-Glucuronidase, a member of the 2 glycosyl hydrolase family, is involved in the hydrolysis of β-glucuronic acid residues from the non-reducing termini of glycosaminoglycans (GAGs). β-Glucuronidase catalyzes the complete hydrolysis of urinary conjugates. β-Glucuronidase serves as a possible candidate enzyme for gene-mediated enzyme prodrug therapy for glucuronide prodrugs. β-glucuronidase-responsive albumin binding prodrugs might be an effective therapeutic practice for treating solid tumors.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will liberate 1.0 µg phenolphthalein from phenolphthalein glucuronide per h at 37°C, pH 5.0.

Physical form

A 65% saturated ammonium sulfate suspension.

Reconstitution

Following reconstitution, store in refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Shibasaki, H., et al. 2001. Steroids66, 795.
Graef, V., et al. 1977. Clin. Chem.23, 532.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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