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MAB1620-AF488

Sigma-Aldrich

Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor 488

clone D5/16B4, from mouse, ALEXA FLUOR 488

Synonym(s):

Keratin, type II cytoskeletal 5, 58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5

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₩1,395,219

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0.1 ML
₩1,395,219

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

₩1,395,219


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biological source

mouse

Quality Level

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

D5/16B4, monoclonal

species reactivity

mouse, human, rat

technique(s)

immunocytochemistry: suitable

isotype

IgG1

NCBI accession no.

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antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

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antibody form

affinity isolated antibody

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buffered aqueous glycerol solution

General description

Keratin, type II cytoskeletal 5 (UniProt P13647; also known as 58 kDa cytokeratin, CK-5, Cytokeratin-5, K5, Keratin-5, Type-II keratin Kb5) is encoded by the KRT5 (also known as DDD1, DM-EBS, EBSMCE, K-EBS, MP-EBS, WC-EBS) gene (Gene ID 3852) in human. Cytokeratin-5 (Keratin-5 or K5) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins. Each epithelium is characterized by its unique keratin expression pattern. In interfollicular epidermis, four major keratins are synthesized and co-polymerize in pairs, K5/K14 and K1/KI0, to form filaments. The expression of these two pairs is strictly regulated during normal keratinocyte differentiation. The K5/K14 filament is expressed in proliferating basal cells, while the K1/K10 filament is expressed during the transition to terminal differentiation and at the cessation of cell division. Similar to other IF members, Keratin-5 contains a central rod region composed of alpha helix segments (a.a. 168-203, 223-315, 339-477) that allow two different IF proteins, one type I (acidic) and one type II (basic) keratin, to intertwine into a coiled-coil formation via hydrophobic interaction. Kearain-5 is a type II keratin that pairs with the type I keratin-14 to form IF.

Application

Anti-Cytokeratin 5,6 Antibody, clone D5/16B4, Alexa Fluor 488 is an antibody against Cytokeratin 5,6 for use in Immunocytochemistry.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Cytokeratin 5/6 in A431 cells.

Target description

Cytokeratin 4 (59 kDa), Cytokeratin 5 (58 kDa) and Cytokeratin 6 (56 kDa).

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Li Su et al.
The Journal of biological chemistry, 278(17), 15232-15238 (2003-02-19)
In the present study, we showed that SPA-1, a Rap1 GTPase-activating protein (GAP), was bound to a cytoskeleton-anchoring protein AF-6. SPA-1 and AF-6 were co-immunoprecipitated in the 293T cells transfected with both cDNAs as well as in normal thymocytes. In
B Rubinfeld et al.
Cell, 65(6), 1033-1042 (1991-06-14)
The rap1/Krev-1 gene encodes a ras-related protein that suppresses transformation by ras oncogenes. We have purified an 88 kd GTPase activating protein (GAP), specific for the rap1/Krev-1 gene product, from bovine brain. Based on partial amino acid sequences obtained from
Oliver Daumke et al.
Nature, 429(6988), 197-201 (2004-05-14)
Rap1 is a Ras-like guanine-nucleotide-binding protein (GNBP) that is involved in a variety of signal-transduction processes. It regulates integrin-mediated cell adhesion and might activate extracellular signal-regulated kinase. Like other Ras-like GNBPs, Rap1 is regulated by guanine-nucleotide-exchange factors (GEFs) and GTPase-activating
H Kurachi et al.
The Journal of biological chemistry, 272(44), 28081-28088 (1997-11-05)
Mouse Spa-1 gene with a region homologous to the human rap1GAP gene is transcriptionally induced in the lymphocytes by mitogenic stimulation. Herein we have cloned a cDNA for its human counterpart. SPA-1 cDNA encodes a 130-kDa protein (p130(SPA-1)) consisting of
James J Hoy et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(10), 13900-13917 (2020-08-25)
The PKA-inhibitor (PKI) family members PKIα, PKIβ, and PKIγ bind with high affinity to PKA and block its kinase activity, modulating the extent, and duration of PKA-mediated signaling events. While PKA is a well-known regulator of physiological and oncogenic events

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