Granulocyte colony-stimulating factor receptor (UniProt Q99062; also known as CD114, G-CSF-R, G-CSF receptor) is encoded by the CSF3R (also known as GCSFR) gene (Gene ID 1441) in human. CD114/G-CSF-R belongs to the type I subgroup of the superfamily of cytokine receptors. G-CSF-R mediates granulocyte proliferation and differentiation in both normal and malignant hematopoiesis. G-CSF-R functions as the receptor for granulocyte colony-stimulating factor (G-CSF) and is activated via ligand-induced homodimerization. The G-CSF/GCSF-R complex mediates survival, proliferation, and maturation of neutrophils and regulates granulopoiesis under both normal and stress conditions. Cell signaling via G-CSF-R is transduced primarily via the Jak/STAT pathway, but the Ras/Raf/MAP kinase and PKB/Akt pathways are also involved. CSF3R gene mutations are well documented in congenital neutropenia as well as the associated predisposition to myelodysplasia and acute myeloid leukemia myeloproliferative disorders. Human G-CSF-R is produced with a signal peptide sequence (a.a. 1-24), the removal of which yields the mature single transmembrane (a.a. 628-650) receptor with a C-terminal cytoplasmic tail (a.a. 651-836) and a large N-terminal extracellular sequence (a.a. 25-627) composed of a C2-type Ig-like domain (a.a. 25-117) and five type-III fibronectin domains (a.a. 125-623).

Clone 5A11.1 was raised against a cytoplasmic domain fragment of the canonical isoform GCSFR-1. Cross-reactivity of this monoclonal antibody toward spliced isoforms GCSFR-2, -3, and -4 is likely, but has not been confirmed.

Epitope: Cytoplasmic domain.

GST-tagged recombinant human G-CSF-R/CD114 cytoplasmic domain fragment.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected G-CSF-R/CD114 in human placenta and bone marrow tissue sections.

Research Category
Apoptosis & Cancer

Research Sub Category
Cytokines & Cytokine Receptors

This Anti-G-CSF-R Antibody, clone 5A11.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of CD114.

Evaluated by Western Blotting of GST-tagged human G-CSF-R cytoplasmic domain fragment.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected 50 ng of GST-tagged recombinant human G-CSF-R cytoplasmic domain fragment.

~43.2 kDa observed for fragment. 89.62 kDa (isoform GCSFR-1), 82.58 kDa (isoform GCSFR-2), 92.58 kDa (isoform GCSFR-3), 84.07 kDa (isoform GCSFR-4; D7) calculated.

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.