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MABC1690M

Sigma-Aldrich

Anti-RAINBOW

from mouse

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5 G
₩203,508

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

₩203,508

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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

packaging

antibody small pack of 25 μL

technique(s)

western blot: suitable

isotype

IgG1κ

target post-translational modification

unmodified

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This Item
186295108146235296
3,5-Bis(trifluoromethyl)phenylacetic acid 98%

263419

3,5-Bis(trifluoromethyl)phenylacetic acid

3-Bromobenzoic acid 98%

186295

3-Bromobenzoic acid

Benzenesulfonamide ≥98%

108146

Benzenesulfonamide

2-(Trifluoromethyl)phenylacetic acid 98%

235296

2-(Trifluoromethyl)phenylacetic acid

assay

98%

assay

98%

assay

≥98%

assay

98%

solubility

methanol: soluble 25 mg/mL, clear, colorless

solubility

methanol: soluble 50 mg/mL, clear, colorless

solubility

methanol: soluble 25 mg/mL

solubility

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

form

solid

form

-

form

solid

form

solid

mp

121-123 °C (lit.)

mp

155-158 °C (lit.)

mp

149-152 °C (lit.)

mp

100-102 °C (lit.)

functional group

carboxylic acid

functional group

bromo, carboxylic acid

functional group

-

functional group

carboxylic acid, fluoro

General description

Anti-RAINBOW Antibody, Cat. No. MABC1690-25UL, validated for use in Western Blotting.
Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

Specificity

Anti-RAINBOW is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.

Immunogen

Remazol-dyes phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

Application

Anti-RAINBOW, Cat. No. MABC1690, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.
Western Blotting Analysis: A representative lot detected Remazol-dye stained protein markers in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.

Quality

Evaluated by Western Blotting in Pre-Stained protein standard. Western Blotting Analysis: A 1:1,000, 1:5,000, and 1:10,000 dilution of this antibody detected Remzaol-dye prestained protein standards .

Physical form

Format: Purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl, pH 7.4, with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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